Changes in brown adipose tissue lipid mediator signatures with aging, obesity, and DHA supplementation in female mice

Brown adipose tissue (BAT) dysfunction in aging and obesity has been related to chronic unresolved inflammation, which could be mediated by an impaired production of specialized proresolving lipid mediators (SPMs), such as Lipoxins-LXs, Resolvins-Rvs, Protectins-PDs, Maresins-MaRs. Our aim was characterize the changes BAT SPMs signatures their association with during aging, especially under obesogenic conditions, modulation a docosahexaenoic acid (DHA)-rich diet. Lipidomic, functional, molecular studies were performed 2- 18-month-old lean (CT) female mice diet-induced obese (DIO) fed high-fat diet (HFD), or DHA-enriched HFD. Aging downregulated Prdm16 UCP1 levels, DIO mice, while DHA partially restored them. Arachidonic (AA)-derived LXs DHA-derived MaRs PDs most abundant young CT mice. Interestingly, sum significantly lower aged compared Some reduced obese-aged included LXB4, MaR2, 4S,14S-diHDHA, 10S,17S-diHDHA (a.k.a. PDX), RvD6. In contrast, increased SPMs, without modifying LXs. However, MicroPET showed that not able counteract cold exposure response data suggest defective underlie decrease activity observed highlight relevance further physiological role therapeutic potential specific on development function.